1xxm

TEM1-β-Lactamase/ β-Lactamase Inhibitor Protein (BLIP)
(For additional information please see TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP))



The enzyme TEM1 β-lactamase (EC 3.5.2.6; TEM1) and its protein inhibitor, β-lactamase inhibitor protein (BLIP) form a complex. The TEM1–BLIP in the KFYEY mutant structure (1xxm) is overlaid on the wildtype complex (1jtg) structure. Mutated TEM1 is shown in red, BLIP (lime) (1xxm), wildtype TEM1 (yellow) and BLIP (1jtg) in  orange , respectively. Mutated BLIP residues (K74A, F142A, Y143A) are colored in blue-violet, TEM1 residues E104 and Y105 , where mutations to alanines were performed (i.g. E104A, Y105A), are colored blue and corresponding to them alanines A104 and A105 are colored <font color='magenta'>magenta in the multiple mutant complex (KFYEY). These two structures are very similar. All-atom RMS deviation (RMSD) between the structures of the wildtype and the KFYEY mutant is 0.37 Å.

About this Structure
1XXM is a Protein complex structure of sequences from Escherichia coli and Streptomyces clavuligerus. Full crystallographic information is available from OCA.

Reference
The modular architecture of protein-protein binding interfaces., Reichmann D, Rahat O, Albeck S, Meged R, Dym O, Schreiber G, Proc Natl Acad Sci U S A. 2005 Jan 4;102(1):57-62. Epub 2004 Dec 23. PMID:15618400

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